Difference between revisions of "Part:BBa K3380630"

 
 
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<partinfo>BBa_K3380630 short</partinfo>
 
<partinfo>BBa_K3380630 short</partinfo>
  
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Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by the FLuoride riboswitch. Upon addition of the fluoride, the riboswitch allows the E.coli sigma 70 polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added.
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===Usage and Biology===
 
===Usage and Biology===
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Can be used in cell-free systems as a biosensor.
  
 
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Latest revision as of 14:54, 27 October 2020


TXO Fluoride riboswitch regulated iSpinach construct under Anderson Promoter (BBa_J23119)

Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by the FLuoride riboswitch. Upon addition of the fluoride, the riboswitch allows the E.coli sigma 70 polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added.


Usage and Biology

Can be used in cell-free systems as a biosensor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]