Difference between revisions of "Part:BBa K3599036"

 
 
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<partinfo>BBa_K3599036 short</partinfo>
 
<partinfo>BBa_K3599036 short</partinfo>
  
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an enzyme that can convert t-anol or isoeugenol to t-anethole or methylisoeugenol, respectively, via methylation of the para-OH group
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An enzyme which was designed into the artificial biosynthesis pathway of 4VA synthesis. The original function of paAIMT was converting t-anol or isoeugenol to t-anethole or methylisoeugenol, respectively, via methylation of the para-OH group. The codon usage was optimized for expression in <i>E. coli</i>.
  
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===Sequence and Features===
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3599036 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3599036 SequenceAndFeatures</partinfo>
  
  
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===Reference===
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===Functional Parameters===
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Koeduka, T., Baiga, T. J., Noel, J. P., & Pichersky, E. (2009). Biosynthesis of t-anethole in anise: characterization of t-anol/isoeugenol synthase and an O-methyltransferase specific for a C7-C8 propenyl side chain. <i>Plant physiology</i>, 149(1), 384-394. [http://www.plantphysiol.org/content/149/1/384.short 10.1104/pp.108.128066]
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<partinfo>BBa_K3599036 parameters</partinfo>
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Latest revision as of 14:44, 27 October 2020


paAIMT

An enzyme which was designed into the artificial biosynthesis pathway of 4VA synthesis. The original function of paAIMT was converting t-anol or isoeugenol to t-anethole or methylisoeugenol, respectively, via methylation of the para-OH group. The codon usage was optimized for expression in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 979
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 979
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 979
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 979
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

Koeduka, T., Baiga, T. J., Noel, J. P., & Pichersky, E. (2009). Biosynthesis of t-anethole in anise: characterization of t-anol/isoeugenol synthase and an O-methyltransferase specific for a C7-C8 propenyl side chain. Plant physiology, 149(1), 384-394. [http://www.plantphysiol.org/content/149/1/384.short 10.1104/pp.108.128066]