Difference between revisions of "Part:BBa K3487005"
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<partinfo>BBa_K3487005 short</partinfo> | <partinfo>BBa_K3487005 short</partinfo> | ||
===Description=== | ===Description=== | ||
| − | RalR acts as a non-specific endonuclease that can cleave methylated and unmethylated DNA, leading to cell death. | + | RalR acts as a non-specific endonuclease that can cleave methylated and unmethylated DNA, then leading to cell death. As a novel type I TA system, RalR is the first toxin that functions as an endonuclease in E. coli. Y.X. Guo,et al found that RalR in E. coli functions differently, acting as a toxin by cleaving DNA, and that it belongs to a type I toxin–antitoxin system. In our project, we used RalR as a toxin protein to cleave the DNA of host cell to avoid the bacterial escape. |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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==2020 SZPT-CHINA== | ==2020 SZPT-CHINA== | ||
===Result=== | ===Result=== | ||
| − | We streaked the engineered strain into a 1Mm IPTG plate for overnight culture, and observed that the engineered strain containing the RalR gene did not grow on the plate. | + | We test the function of this toxin protein in E. coli.We streaked the engineered strain into a 1Mm IPTG plate for overnight culture, and observed that the engineered strain containing the RalR gene did not grow on the plate. |
| − | The expression of RalR was good under the positive and negative controls, and the colony situation in the corresponding area was significantly less than that of the empty vector control group. The result is | + | The expression of RalR was good under the positive and negative controls, and the colony situation in the corresponding area was significantly less than that of the empty vector control group. The result is as follow. |
[[File:T--SZPT-China--RalR.png|center|500px|]] | [[File:T--SZPT-China--RalR.png|center|500px|]] | ||
Revision as of 14:43, 27 October 2020
Non-specific endonuclease
Description
RalR acts as a non-specific endonuclease that can cleave methylated and unmethylated DNA, then leading to cell death. As a novel type I TA system, RalR is the first toxin that functions as an endonuclease in E. coli. Y.X. Guo,et al found that RalR in E. coli functions differently, acting as a toxin by cleaving DNA, and that it belongs to a type I toxin–antitoxin system. In our project, we used RalR as a toxin protein to cleave the DNA of host cell to avoid the bacterial escape.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
2020 SZPT-CHINA
Result
We test the function of this toxin protein in E. coli.We streaked the engineered strain into a 1Mm IPTG plate for overnight culture, and observed that the engineered strain containing the RalR gene did not grow on the plate. The expression of RalR was good under the positive and negative controls, and the colony situation in the corresponding area was significantly less than that of the empty vector control group. The result is as follow.
References
Guo Y, Quiroga C, Chen Q, et al. RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli. Nucleic Acids Res. 2014;42(10):6448-6462.