Difference between revisions of "Part:BBa K3506099"
 
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<b><font size"3">Biology and Usage</font></b>
 
<b><font size"3">Biology and Usage</font></b>
  
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A lot of attention has been drawn to Glyceraldehyde-3-Phosphate Dehydrogenase <i>(GAPDH)</i> promoter. <i>GAPDH</i> mRNA accounts for 2–5% of the poly(A) RNA in yeasts, suggesting that it can be effectively used in the high-efficiency expression of transgenes.[1] In our project, <i>GAPDH</i> promoter is used to effectively transcribe the genes that contains <i>U6</i> promoter and homing guide RNA (hgRNA). General vectors have been designed to rely on homologous <i>GAPDH</i> promoter regions to drive the expressions of heterologous genes in yeasts and other filamentous fungi.
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A lot of attention has been drawn to Glyceraldehyde-3-Phosphate Dehydrogenase <i>(GAPDH)</i> promoter. <i>GAPDH</i> mRNA accounts for 2–5% of the poly(A) RNA in yeasts, suggesting that it can be effectively used in the high-efficiency expression of transgenes[1]. In our project, <i>GAPDH</i> promoter is used to effectively transcribe the genes that contains <i>U6</i> promoter and homing guide RNA (hgRNA). General vectors have been designed to rely on homologous <i>GAPDH</i> promoter regions to drive the expressions of heterologous genes in yeasts and other filamentous fungi.
  
 
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Latest revision as of 14:35, 27 October 2020


GAPDH promoter

Generally, GAPDH promoter can drive the expression of glyceraldehyde-3-phosphate dehydrogenase gene in many species.


Biology and Usage

A lot of attention has been drawn to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) promoter. GAPDH mRNA accounts for 2–5% of the poly(A) RNA in yeasts, suggesting that it can be effectively used in the high-efficiency expression of transgenes[1]. In our project, GAPDH promoter is used to effectively transcribe the genes that contains U6 promoter and homing guide RNA (hgRNA). General vectors have been designed to rely on homologous GAPDH promoter regions to drive the expressions of heterologous genes in yeasts and other filamentous fungi.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 436


Reference

[1]Jia Y, Li S, Allen G, Feng S, Xue L. A novel glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter for expressing transgenes in the halotolerant alga Dunaliella salina. Curr Microbiol. 2012 May;64(5):506-13.