Difference between revisions of "Part:BBa K3468074"
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This mutant increases a salt bridge between E280 and R285. Salt bridge is a kind of electric interaction. | This mutant increases a salt bridge between E280 and R285. Salt bridge is a kind of electric interaction. | ||
+ | We use FoldX to judge the thermal stability of D220E and Jmol to find whether there is a new salt bridge. | ||
+ | [[File:D220E.png|400px|thumb|left|Fig.1 D220E in PyMOL]] | ||
+ | |||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K3468074 parameters</partinfo> | <partinfo>BBa_K3468074 parameters</partinfo> | ||
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Latest revision as of 14:01, 27 October 2020
PETase D220E
The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from D to E at 220 position which can be more stable in higher temperature compared with the wild type.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
This mutant increases a salt bridge between E280 and R285. Salt bridge is a kind of electric interaction. We use FoldX to judge the thermal stability of D220E and Jmol to find whether there is a new salt bridge.