Difference between revisions of "Part:BBa K3468070"

 
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<partinfo>BBa_K3468070 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3468070 SequenceAndFeatures</partinfo>
 
Adding hydrogen bonds  
 
Adding hydrogen bonds  
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The reduction of DDG was predicted by FoldX, and PyOML was used for visual screening. it formed hydrogen bonds with 273-CYS, 274-GLU and 291-LEU, which was mutated into GLN, which was beneficial to improve the thermal stability of PETase.
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[[File:T270Q.png|400px|thumb|left|Fig.1 T270Q in PyMOL]]
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 13:57, 27 October 2020


PETase T270Q

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from T to Q at 270 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Adding hydrogen bonds

The reduction of DDG was predicted by FoldX, and PyOML was used for visual screening. it formed hydrogen bonds with 273-CYS, 274-GLU and 291-LEU, which was mutated into GLN, which was beneficial to improve the thermal stability of PETase.

Fig.1 T270Q in PyMOL