Difference between revisions of "Part:BBa K3365013:Design"
Little ghost (Talk | contribs) (→References) |
|||
| (One intermediate revision by one other user not shown) | |||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| + | |||
The araC regulatory gene is transcribed in the opposite direction. | The araC regulatory gene is transcribed in the opposite direction. | ||
===Source=== | ===Source=== | ||
| − | + | PCR from pS8k-dCas9 given by Prof. Yi Xiao. | |
===References=== | ===References=== | ||
[1] Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30. | [1] Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30. | ||
Latest revision as of 13:57, 27 October 2020
Inducible pBAD/araC promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
The araC regulatory gene is transcribed in the opposite direction.
Source
PCR from pS8k-dCas9 given by Prof. Yi Xiao.
References
[1] Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30.