Difference between revisions of "Part:BBa K3544000:Design"
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===Source=== | ===Source=== | ||
| − | The original nucleotide sequence is from NCBI <i>Botrytis cinerea B05.10</i> chromosome 8, NC_037317.1. The sequence is optimized according to the condon usage bias in <i> | + | The original nucleotide sequence is from NCBI <i>Botrytis cinerea B05.10</i> chromosome 8, NC_037317.1. The sequence is optimized according to the condon usage bias in <i>Saccharomyces cerevisiae</i> and synthesized from Sangon |
===References=== | ===References=== | ||
| + | Siewers V, Kokkelink L, Smedsgaard J, et al. Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea[J]. Applied and Environmental Microbiology, 2006, 72(7): 4619-4626. | ||
Latest revision as of 13:50, 27 October 2020
Coding sequence of BcABA1, a cytochrome P450s belong to biosynthetic genecluster of ABA in B.cinerea
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 438
Illegal PstI site found at 404 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 404
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 438
Illegal PstI site found at 404 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 438
Illegal PstI site found at 404 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1107
Design Notes
An enzyme involved in the synthesis of ABA in Botrytis cinerea. It can catalyze alpha-ionylideneethane into alpha-ionylideneacetic acid
Source
The original nucleotide sequence is from NCBI Botrytis cinerea B05.10 chromosome 8, NC_037317.1. The sequence is optimized according to the condon usage bias in Saccharomyces cerevisiae and synthesized from Sangon
References
Siewers V, Kokkelink L, Smedsgaard J, et al. Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea[J]. Applied and Environmental Microbiology, 2006, 72(7): 4619-4626.