Difference between revisions of "Part:BBa K2904111"
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===Design=== | ===Design=== | ||
It is very important for us to select the inducible promoter with low transcriptional leakage and high dynamic range. These promoters are essential parts of building the logic gates. These promoters also played a crucial role in the subsequent construction of multiple-input logic gates and swapping gates. | It is very important for us to select the inducible promoter with low transcriptional leakage and high dynamic range. These promoters are essential parts of building the logic gates. These promoters also played a crucial role in the subsequent construction of multiple-input logic gates and swapping gates. | ||
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===Result=== | ===Result=== | ||
https://2020.igem.org/wiki/images/thumb/8/86/T--OUC-China--result_fig1A_pTet.jpg/800px-T--OUC-China--result_fig1A_pTet.jpg | https://2020.igem.org/wiki/images/thumb/8/86/T--OUC-China--result_fig1A_pTet.jpg/800px-T--OUC-China--result_fig1A_pTet.jpg | ||
| − | The Tet promoter fluorescence data in BL21 (DE3) at different aTc concentrations (0mg/ | + | The Tet promoter fluorescence data in <span style="font-style: italic;">E. coli</span> BL21 (DE3) at different aTc concentrations (0mg/mL, 0.01mg/mL, 0.1mg/mL, 0.25mg/mL, 0.5mg/mL and 1mg/mL). The Tet promoter showed the highest fluorescence intensity when the aTc concentration was 0.25mg/mL. |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 13:49, 27 October 2020
aTc inducible Tet promoter
This promoter is induced by aTc. It was used by 2019 OUC-China team.
OUC-China 2020’s characterization
Design
It is very important for us to select the inducible promoter with low transcriptional leakage and high dynamic range. These promoters are essential parts of building the logic gates. These promoters also played a crucial role in the subsequent construction of multiple-input logic gates and swapping gates.
Result
The Tet promoter fluorescence data in E. coli BL21 (DE3) at different aTc concentrations (0mg/mL, 0.01mg/mL, 0.1mg/mL, 0.25mg/mL, 0.5mg/mL and 1mg/mL). The Tet promoter showed the highest fluorescence intensity when the aTc concentration was 0.25mg/mL.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]