Difference between revisions of "Part:BBa K3605001"

Revision as of 13:39, 27 October 2020

Glucose hexokinase (GHK) from Gluconacetobacter xylinus

Glucose hexokinase (GHK) was originated from Gluconacetobacter xylinus CGMCC 2955. Using glucose, this enzyme catalyzes the formation of glucose-6-phosphate, which is the glycolytic intermediate and the origin of substrate synthesis for Bacterial cellulose production.

Before we clone this gene,the software SnapGene viewer was used to analyze the sequence of GHK, its feature is showed as follows:

Glucose hexokinase (GHK) was amplified using PCR method, and inserted into the vector pSB1C3. The result was identified in figure 1.

Fig.1. The result of Glucose hexokinase (GHK) gene cloning. M: Marker; 1: PCR result of GHK; 2: Digestion of pSB1C3 containing GHK.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 548
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 478

@@ Line 4: / Line 4: @@
 Glucose hexokinase (GHK) was originated from Gluconacetobacter xylinus CGMCC 2955. Using glucose, this enzyme catalyzes the formation of glucose-6-phosphate, which is the glycolytic intermediate and the origin of substrate synthesis for Bacterial cellulose production.
+<br/>
+[[File:K3605001-1.jpg|center]]
+<br/>
+<br/>
+Before we clone this gene,the software SnapGene viewer was used to analyze the sequence of GHK, its feature is showed as follows:
+<br/>
+[[File:K3605001-2.jpg|center]]
+<br/>
+<br/>
+Glucose hexokinase (GHK) was amplified using PCR method, and inserted into the vector pSB1C3. The result was identified in figure 1.
+<br/>
+[[File:K3605001-3.jpg|center]]
+Fig.1. The result of Glucose hexokinase (GHK) gene cloning.
+M: Marker; 1: PCR result of GHK; 2: Digestion of pSB1C3 containing GHK.
 <!-- Add more about the biology of this part here