Difference between revisions of "Part:BBa K3468048"

Latest revision as of 13:35, 27 October 2020

PETase S207P

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from S to P at 207 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

This mutant undergoes proline substitution. Proline has a special ring structure, which can increase the rigidity of the loop, reduce the conformational entropy, and improve the thermal stability of the protein.

Using FoldX and I-Mutant to predict the stability of S142P, it was found that the stability decreased, and the result was not ideal. Because Ser207 is very close to the active site of PETase, the introduction of proline will reduce the enzyme activity. A40P

@@ Line 11: / Line 11: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3468048 SequenceAndFeatures</partinfo>
+This mutant undergoes proline substitution. Proline has a special ring structure, which can increase the rigidity of the loop, reduce the conformational entropy, and improve the thermal stability of the protein.
+Using FoldX and I-Mutant to predict the stability of S142P, it was found that the stability decreased, and the result was not ideal. Because Ser207 is very close to the active site of PETase, the introduction of proline will reduce the enzyme activity.
+A40P
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K3468048 parameters</partinfo>
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