Difference between revisions of "Part:BBa K3468037"
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3468037 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3468037 SequenceAndFeatures</partinfo>
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===Applications of BBa_K3468037===
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The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bonds with 152-ALA itself and ASP-150. after mutating it into SER, it also formed hydrogen bonds with LYS-177, which stabilized α3-β5loop and α4-β6loop, which was beneficial to improve the thermal stability of PETase.
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[[File:Ala152.png|400px|thumb|left|Fig.1 Ala152 in PyMOL]]
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[[File:A152N.png|400px|thumb|left|Fig.2 A152N in PyMOL]]
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Adding hydrogen bonds stabilized α3-β5loop and α4-β6loop
 
Adding hydrogen bonds stabilized α3-β5loop and α4-β6loop
  

Latest revision as of 13:23, 27 October 2020


PETase A152N

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from A to N at 152 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Applications of BBa_K3468037

The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bonds with 152-ALA itself and ASP-150. after mutating it into SER, it also formed hydrogen bonds with LYS-177, which stabilized α3-β5loop and α4-β6loop, which was beneficial to improve the thermal stability of PETase.

Fig.1 Ala152 in PyMOL
Fig.2 A152N in PyMOL

Adding hydrogen bonds stabilized α3-β5loop and α4-β6loop