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===Usage and Biology===
 
===Usage and Biology===
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We use FoldX to judge the thermal stability of T116R and Jmol to find whether there is a new salt bridge. Finally we use Pymol to find out what interaction make it become stable.
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[[File:T116R.png|400px|thumb|left|Fig.1 T116R in PyMOL]]
  
 
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Revision as of 13:08, 27 October 2020


PETase T116R

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from T to R at 116 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

There is new hydrogen bond increased between T116R and T88, The hydrogen bond is an interaction formed by N, O and H in a protein, which can stabilize the structure of the protein and improve thermal stability.