Difference between revisions of "Part:BBa K3468033"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | We use FoldX to judge the thermal stability of S278D and Jmol to find whether there is a new salt bridge. | ||
| + | [[File:S278D.png|400px|thumb|left|Fig.1 S278D in PyMOL]] | ||
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<partinfo>BBa_K3468033 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3468033 SequenceAndFeatures</partinfo> | ||
| − | + | This mutant increases a salt bridge between S278D and R280. Salt bridge is a kind of electric interaction. | |
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K3468033 parameters</partinfo> | <partinfo>BBa_K3468033 parameters</partinfo> | ||
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Latest revision as of 13:06, 27 October 2020
PETase S278D
The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from S to D at 278 position which can be more stable in higher temperature compared with the wild type.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
This mutant increases a salt bridge between S278D and R280. Salt bridge is a kind of electric interaction.