Difference between revisions of "Part:BBa K3328031"

Latest revision as of 13:05, 27 October 2020

GFPmut3b-ASV

GFPmut3b protein with ASV-tagged whose half-life is ~110 min

Due to its rapid degradation, it showed advantages in our 3WJ characterization. When a complementary trigger RNA is expressed, the trigger will bind to the switch RNA, making the originally unstable 3WJ structure stable, and represses translation. This avoids weakening the inhibitory effect due to the accumulation of GFP.

In the validation of 3WJ, as with toehold, we also set blank control (IPTG=0 M, aTc=0 mg/mL). Compared with the group without trigger expression (IPTG=0.1 M, aTc=0 mg/mL), the group with trigger expression (IPTG=0.1 M, aTc=0.25 mg/mL) showed inhibitory effect. Error bar: SD (n=9).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 644

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3328031 short</partinfo>
+GFPmut3b protein with ASV-tagged whose half-life is ~110 min
+Due to its rapid degradation, it showed advantages in our 3WJ characterization. When a complementary trigger RNA is expressed, the trigger will bind to the switch RNA, making the originally unstable 3WJ structure stable, and represses translation. This avoids weakening the inhibitory effect due to the accumulation of GFP.
+https://2020.igem.org/wiki/images/thumb/c/c6/T--OUC-China--result_fig3.jpg/653px-T--OUC-China--result_fig3.jpg
+In the validation of 3WJ, as with toehold, we also set blank control (IPTG=0 M, aTc=0 mg/mL). Compared with the group without trigger expression (IPTG=0.1 M, aTc=0 mg/mL), the group with trigger expression (IPTG=0.1 M, aTc=0.25 mg/mL) showed inhibitory effect. Error bar: SD (n=9).
 <!-- Add more about the biology of this part here