Difference between revisions of "Part:BBa K3365053:Design"
| (One intermediate revision by the same user not shown) | |||
| Line 8: | Line 8: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | Be careful especially when | + | Be careful especially when design primers. Due to the complex secondary structure of double terminator, we put a small nonfunctional sequence downstream of the double terminator as linker for the convenience of PCR. |
| − | + | ||
===Source=== | ===Source=== | ||
| − | We amplify this part on plasmid pE1a-RFP | + | We amplify this part on plasmid pE1a-RFP, which is offered by team Tongji_China. |
===References=== | ===References=== | ||
Latest revision as of 12:56, 27 October 2020
RFP with double terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 700
Illegal XhoI site found at 691 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 555
Illegal AgeI site found at 667 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Be careful especially when design primers. Due to the complex secondary structure of double terminator, we put a small nonfunctional sequence downstream of the double terminator as linker for the convenience of PCR.
Source
We amplify this part on plasmid pE1a-RFP, which is offered by team Tongji_China.