Difference between revisions of "Part:BBa K3544000:Design"

(→‎Design Notes)
(→‎Source)
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===Source===
 
===Source===
  
−
Escherichia coli
+
The original nucleotide sequence is from NCBI <i>Botrytis cinerea B05.10</i> chromosome 8, NC_037317.1. The sequence is optimized according to the condon usage bias in <i>S.c</i> and synthesized from Sangon
  
 
===References===
 
===References===

Revision as of 12:55, 27 October 2020


Coding sequence of BcABA1, a cytochrome P450s belong to biosynthetic genecluster of ABA in B.cinerea


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 438
    Illegal PstI site found at 404
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 404
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 438
    Illegal PstI site found at 404
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 438
    Illegal PstI site found at 404
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1107


Design Notes

An enzyme involved in the synthesis of ABA in Botrytis cinerea. It can catalyze alpha-ionylideneethane into alpha-ionylideneacetic acid

Source

The original nucleotide sequence is from NCBI Botrytis cinerea B05.10 chromosome 8, NC_037317.1. The sequence is optimized according to the condon usage bias in S.c and synthesized from Sangon

References