Difference between revisions of "Part:BBa K133051"

 
 
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<partinfo>BBa_K133051 short</partinfo>
 
<partinfo>BBa_K133051 short</partinfo>
  
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TetR repressible promoter with ribosomal binding site. Chimeric protein fused with Red Flourescent Protein for detecting protein localisation. Added RGD peptide improves internalization. Hexa hystidine tag serves for: protein isolation with NiNTA or specific AntiHis antibody labelling.
  
  

Latest revision as of 00:21, 30 October 2008

TetR-CF-mCherry-RGD-Histop

TetR repressible promoter with ribosomal binding site. Chimeric protein fused with Red Flourescent Protein for detecting protein localisation. Added RGD peptide improves internalization. Hexa hystidine tag serves for: protein isolation with NiNTA or specific AntiHis antibody labelling.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1267
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 824
    Illegal BamHI site found at 1335
    Illegal BamHI site found at 2299
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 361
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2334