Difference between revisions of "Part:BBa K3365056:Design"

 
 
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===Design Notes===
 
===Design Notes===
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In order to make the same distance between transcription initiation site and target sequence, we add one more base pair to spacer as the lure sequences contain only 20 base pair.
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The lure we designed is 20 bp long while the target is 21 bp long. In order to keep the distance between transcription initiation site and target sequence consistent, we add one more base pair to spacer of lure .
  
  

Latest revision as of 12:35, 27 October 2020


Second potential off-target site for dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 66
    Illegal NheI site found at 89
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The lure we designed is 20 bp long while the target is 21 bp long. In order to keep the distance between transcription initiation site and target sequence consistent, we add one more base pair to spacer of lure .


Source

We get this lure sequence from a study on refractory non-small-cell lung cancer.

References