Difference between revisions of "Part:BBa K079045"
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| − | {| cellpadding=" | + | {| cellpadding="15" border="0" align="center" |
| − | |[[Image:LacPlasmid.jpg |left|500 px ]] | + | |rowspan=5|[[Image:LacPlasmid.jpg |left|500 px ]] |
|'''<font size="5">LAC LIBRARY</font>''' | |'''<font size="5">LAC LIBRARY</font>''' | ||
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| + | | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079018 Lac1] | ||
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| + | | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079019 Lac2] | ||
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| + | | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079017 Lac SymL] | ||
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| − | |[[Image:Tet.jpg |right|500 px ]] | + | | |
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| + | |rowspan=5|[[Image:Tet.jpg |right|500 px ]] | ||
|'''<font size="5">TET LIBRARY</font>''' | |'''<font size="5">TET LIBRARY</font>''' | ||
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| − | |[[Image: | + | | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079036 TeT O] |
| + | |- | ||
| + | | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079037 TetO-4C] | ||
| + | |- | ||
| + | | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079037 TetO-wt/4C5G] | ||
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| + | |rowspan=5|[[Image:CI.jpg |right|500 px ]] | ||
|'''<font size="5">LAMBDA LIBRARY</font>''' | |'''<font size="5">LAMBDA LIBRARY</font>''' | ||
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| − | |[[Image: | + | | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079041 Lambda OR1] |
| + | |- | ||
| + | | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079042 Lambda OR2] | ||
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| + | | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079043 Lambda OR3] | ||
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| + | |rowspan=5|[[Image:LexLib.jpg |right|500 px ]] | ||
|'''<font size="5">LEXA LIBRARY</font>''' | |'''<font size="5">LEXA LIBRARY</font>''' | ||
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| − | + | | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079039 LexA 1] | |
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| + | | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079040 LexA 2] | ||
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| − | + | Right now we're ultimating the design and testing of an equally [http://2008.igem.org/Team:Bologna/Wetlab#Operator_site_cloning_in_standard_plasmids simple protocol] to transfer operator sequences in Biobrick Standard Assembly Vectors using standard cloning reagents and avoiding as much as possible experimentally fragile or optimization dependent steps. | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 00:19, 30 October 2008
Lac operator library
Parts K079045 through K079048 are libraries intended to introduce standardization and modularity for operator sequences.
Each of the 4 collections has a different repressor specificity (Lac, Tet, Lambda and LexA) and consists of 3 different members with distinct binding affinities.
Since operators flanked by Biobrick ends are unpractical and costly to obtain, both by dna sinthesys and phosphorylated oligos, we designed a "Standard Collection Vector".
This layout allows to get multiple small sequences with a single synthesis process and specifically enable the extraction of one or a combination of operators using exclusively Biobrick prefix and suffix restriction enzymes. Digestion and religation of the collection vector with Xba and Pst yields a single Operators 1 and Operator 3 vectors respectively (Es: Lac2 and Lac Symmetric) avoiding the troubles and optimization related to the run and purification of very small bands on agarose gel.
A similar approach using EcoRI give rise to a multiple Operator Vector comprising Operator 1 and Operator 2 sequences, usable per se as a composite regulatory site or further restrictable with PstI to extract the single Operator 2 (Es: Lac1)
The Libraries have been syntetized by Geneart in their standard pGA18 and pMA synthesis vector, with both ColE1 high copy number origin of replication and Ampicillin resistance.
 |
LAC LIBRARY |
| Lac1 | |
| Lac2 | |
| Lac SymL | |
 |
TET LIBRARY |
| TeT O | |
| TetO-4C | |
| TetO-wt/4C5G | |
 |
LAMBDA LIBRARY |
| Lambda OR1 | |
| Lambda OR2 | |
| Lambda OR3 | |
 |
LEXA LIBRARY |
| LexA 1 | |
| LexA 2 | |
Right now we're ultimating the design and testing of an equally [http://2008.igem.org/Team:Bologna/Wetlab#Operator_site_cloning_in_standard_plasmids simple protocol] to transfer operator sequences in Biobrick Standard Assembly Vectors using standard cloning reagents and avoiding as much as possible experimentally fragile or optimization dependent steps.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]