Difference between revisions of "Part:BBa K3416029"
WiseauTommy (Talk | contribs) |
|||
| Line 1: | Line 1: | ||
| + | =Introduction= | ||
| + | [[File:T--Vilnius-Lithuania--FFlogo.png|80px|right|FlavoFlow]] | ||
| − | + | Vilnius-Lithuania iGEM 2020 project [https://2020.igem.org/Team:Vilnius-Lithuania <b>FlavoFlow]</b>includes three goals towards looking for <i>Flavobacterium</i> disease-related problems’ solutions. The project includes creating a rapid detection kit, based on HDA and LFA, developing an implement for treating a disease, and introducing the foundation of edible vaccines. | |
| − | < | + | This part was used for the second goal- treatment - of the project FlavoFlow. |
| − | + | ==Biology== | |
| − | + | Bacteriophages or any other virus can inject its genomic material into the host cell environment. But to achieve it virus needs to create a passage throughout diverse carbohydrate barriers such as O-polysaccharide chains of lipopolysaccharide molecules, capsule polysaccharides, peptidoglycan layers, or even extracellular polysaccharides which can form biofilm matrix<sup>1</sup>. | |
| − | + | That is why viruses have a big arsenal of proteins, mostly located on its tail spikes, which can penetrate this polysaccharide obstacle. Sometimes, the amount of these proteins is the only factor that defines bacteriophage’s host spectrum and specificity<sup>2</sup>. | |
| − | == | + | Exolysins are also called depolymerases which are a component of the adsorption apparatus, most commonly with two domains essential for viral infection. The first domain responsible for initiating binding to the capsule and the second one is responsible for digestion of the capsule. Biochemically speaking, depolymerases could be one of two groups - lyases or hydrolases. The biggest difference between them is that when lyase cleaves a specific bond of a substrate, it does not consume water molecules from the environment. That could be a significant advantage if the enzyme should catalyze the reaction in a waterless environment. |
| − | + | Exolysins <b>gp529</b> (BBa_K3416029), <b>gp531</b> ([https://parts.igem.org/Part:BBa_K3416031 BBa_K3416031),] <b>gp533</b> ([https://parts.igem.org/Part:BBa_K3416033 BBa_K3416033)] belong to the giant singleton phage RAK-2 which infects multidrug-resistant <i>Klebsiella pneumoniae</i> KV-3 2020 [https://2020.igem.org/Team:Vilnius-Lithuania Vilnius-Lithuania iGEM team]used synthetic biology to design a construct of an [https://2020.igem.org/Team:Vilnius-Lithuania/Design#headingTreatment AI-2 induced exolysin-endolysin system]. | |
| − | < | + | |
| − | < | + | |
| − | < | + | |
| − | + | ||
| − | + | ||
| − | < | + | |
| − | + | ||
| − | < | + | |
| − | + | ||
Revision as of 11:30, 27 October 2020
Introduction
Vilnius-Lithuania iGEM 2020 project FlavoFlowincludes three goals towards looking for Flavobacterium disease-related problems’ solutions. The project includes creating a rapid detection kit, based on HDA and LFA, developing an implement for treating a disease, and introducing the foundation of edible vaccines. This part was used for the second goal- treatment - of the project FlavoFlow.
Biology
Bacteriophages or any other virus can inject its genomic material into the host cell environment. But to achieve it virus needs to create a passage throughout diverse carbohydrate barriers such as O-polysaccharide chains of lipopolysaccharide molecules, capsule polysaccharides, peptidoglycan layers, or even extracellular polysaccharides which can form biofilm matrix1. That is why viruses have a big arsenal of proteins, mostly located on its tail spikes, which can penetrate this polysaccharide obstacle. Sometimes, the amount of these proteins is the only factor that defines bacteriophage’s host spectrum and specificity2. Exolysins are also called depolymerases which are a component of the adsorption apparatus, most commonly with two domains essential for viral infection. The first domain responsible for initiating binding to the capsule and the second one is responsible for digestion of the capsule. Biochemically speaking, depolymerases could be one of two groups - lyases or hydrolases. The biggest difference between them is that when lyase cleaves a specific bond of a substrate, it does not consume water molecules from the environment. That could be a significant advantage if the enzyme should catalyze the reaction in a waterless environment. Exolysins gp529 (BBa_K3416029), gp531 (BBa_K3416031), gp533 (BBa_K3416033) belong to the giant singleton phage RAK-2 which infects multidrug-resistant Klebsiella pneumoniae KV-3 2020 Vilnius-Lithuania iGEM teamused synthetic biology to design a construct of an AI-2 induced exolysin-endolysin system.