Difference between revisions of "Part:BBa K3328032"
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<partinfo>BBa_K3328032 short</partinfo> | <partinfo>BBa_K3328032 short</partinfo> | ||
− | + | realize the boolean calculation of XOR | |
+ | ===Design=== | ||
+ | The XOR is inspired by the NIMPLY gate, consisting of a toehold switch and two triggers. The trigger’s core sequence is the same and at the triggers’ both ends there are the nucleotide-binding domains. When input one of these triggers, the switch can turn on. Moreover, when input these two triggers simultaneously, they can pair together and form a ring in the middle. As a result, the switch will still be in the OFF state. | ||
− | + | https://2020.igem.org/wiki/images/thumb/9/99/T--OUC-China--design_lunbo_xor.jpg/799px-T--OUC-China--design_lunbo_xor.jpg | |
− | === | + | |
+ | ===Result=== | ||
+ | https://2020.igem.org/wiki/images/thumb/7/70/T--OUC-China--part_XOR1.jpg/800px-T--OUC-China--part_XOR1.jpg | ||
+ | The left figure shows that when INPUT A and INPUT B are both 0 or 1, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other two groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9). | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3328032 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3328032 SequenceAndFeatures</partinfo> | ||
+ | |||
Latest revision as of 11:26, 27 October 2020
switch of XOR gate (XOR1)
realize the boolean calculation of XOR
Design
The XOR is inspired by the NIMPLY gate, consisting of a toehold switch and two triggers. The trigger’s core sequence is the same and at the triggers’ both ends there are the nucleotide-binding domains. When input one of these triggers, the switch can turn on. Moreover, when input these two triggers simultaneously, they can pair together and form a ring in the middle. As a result, the switch will still be in the OFF state.
Result
The left figure shows that when INPUT A and INPUT B are both 0 or 1, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other two groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]