Difference between revisions of "Part:BBa K3407018"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3407018 short</partinfo>
 
<partinfo>BBa_K3407018 short</partinfo>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Usage and Biology===
 
===Usage and Biology===
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This part was used to Express
+
This part was used to Express mini-3 from <i>B. subtilis</i> (<html><a href="https://parts.igem.org/Part:BBa_K3407002" target="_blank"><b>BBa_K3407002</b></a></html>)

Revision as of 11:16, 27 October 2020


Mini-3 endoribonuclease with araBAD promoter - RBS - and rrnBT1 terminator

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 300
    Illegal XbaI site found at 673
    Illegal PstI site found at 527
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 300
    Illegal PstI site found at 527
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 300
    Illegal BglII site found at 309
    Illegal BamHI site found at 809
    Illegal XhoI site found at 818
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 300
    Illegal XbaI site found at 673
    Illegal PstI site found at 527
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 300
    Illegal XbaI site found at 673
    Illegal PstI site found at 527
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56

Usage and Biology

This part was used to Express mini-3 from B. subtilis (BBa_K3407002)