Difference between revisions of "Part:BBa K3328026"
(→Result) |
|||
(4 intermediate revisions by 2 users not shown) | |||
Line 3: | Line 3: | ||
<partinfo>BBa_K3328026 short</partinfo> | <partinfo>BBa_K3328026 short</partinfo> | ||
− | two input | + | two input triggers of NIMPLY2 logic gate to perform the truth table |
+ | |||
+ | ===Design=== | ||
+ | In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on. | ||
+ | |||
+ | https://2020.igem.org/wiki/images/thumb/8/87/T--OUC-China--design_lunbo_nimply.jpg/799px-T--OUC-China--design_lunbo_nimply.jpg | ||
+ | |||
+ | ===Result=== | ||
+ | https://2020.igem.org/wiki/images/thumb/1/1f/T--OUC-China--part_NIMPLY2.jpg/800px-T--OUC-China--part_NIMPLY2.jpg | ||
+ | |||
+ | The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9). | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 11:13, 27 October 2020
triggers of NIMPLY gate (NIMPLY2)
two input triggers of NIMPLY2 logic gate to perform the truth table
Design
In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on.
Result
The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 110
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 107
Illegal XhoI site found at 1 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 110
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 110
- 1000COMPATIBLE WITH RFC[1000]