Difference between revisions of "Part:BBa K3328022"
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<partinfo>BBa_K3328022 short</partinfo> | <partinfo>BBa_K3328022 short</partinfo> | ||
| − | two input | + | two input triggers of AND logic gate to perform the truth table |
===Design=== | ===Design=== | ||
AND gate constructed from two input RNAs that bind to yield a complete trigger RNA. When either input RNA is expressed, it is incapable of activating the switch because neither trigger sub-sequence alone can unwind the repressing hairpin. The toehold switch can only be turned on when the two input RNA species hybridize and form a complete trigger sequence. | AND gate constructed from two input RNAs that bind to yield a complete trigger RNA. When either input RNA is expressed, it is incapable of activating the switch because neither trigger sub-sequence alone can unwind the repressing hairpin. The toehold switch can only be turned on when the two input RNA species hybridize and form a complete trigger sequence. | ||
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https://2020.igem.org/wiki/images/thumb/6/66/T--OUC-China--part_AND.jpg/800px-T--OUC-China--part_AND.jpg | https://2020.igem.org/wiki/images/thumb/6/66/T--OUC-China--part_AND.jpg/800px-T--OUC-China--part_AND.jpg | ||
| − | The left figure shows that when INPUT A=1, INPUT B=1, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ | + | The left figure shows that when INPUT A=1, INPUT B=1, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9). |
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Latest revision as of 11:03, 27 October 2020
triggers of AND gate
two input triggers of AND logic gate to perform the truth table
Design
AND gate constructed from two input RNAs that bind to yield a complete trigger RNA. When either input RNA is expressed, it is incapable of activating the switch because neither trigger sub-sequence alone can unwind the repressing hairpin. The toehold switch can only be turned on when the two input RNA species hybridize and form a complete trigger sequence.
Result
The left figure shows that when INPUT A=1, INPUT B=1, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]