Difference between revisions of "Part:BBa K3447000"
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<partinfo>BBa_K3447000 short</partinfo>
 
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Colicin V is a non-ribosome-encoded antibacterial peptide produced by many <i>E. coli</i> strains and other members of the Enterobacteriaceae family, which kills bacteria by destroying the membrane potential of sensitive target cells. Due to the existence and composition of the outer membrane of Gram-negative bacteria, natural colicin V is usually inactive to it, but its expression in lactic acid bacteria has also been proposed in the literature: naturally generated colicin V can destroy sensitive targets Cell membrane potential to kill Gram-positive bacteria. The gene encoding colicin V is derived from the pAPEC-O2-ColV plasmid of <i>E. coli</i>.<br><br>
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The <i>cvaA</i> and <i>cvaB</i> genes encode components of a dedicated secretion system required for extracellular secretion of colistin. The CvaB protein encoded by the <i>cvaB</i> gene is the central membrane transporter of the bacteriocin V secretion system in <i>Escherichia coli</i>. CvaB, together with CvaA and TolC proteins, direct the secretion of colicin V through the cleavage of the bisglycine leader peptide. The sequence alignment shows that CvaB protein belongs to the ABC superfamily.
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LuxI is the synthetase of AHL from <i>Vibrio fischeri</i>.<br>
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===Usage and Biology===
 
===Usage and Biology===
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LuxI gene is an important gene for gram-negative bacteria to conduct quorum sensing communication. It is responsible for the synthesis of AHL signaling molecules. AHL, an acyl hyperserine lactone, is the main signal molecule for gram-negative bacteria to communicate quorum sensing information. <i>E. coli</i> is a typical Gram-negative bacterium, but its quoral-sensing system 1 is not complete. It lacks the luxI gene, so it cannot synthesize AHL synthase and thus AHL signal molecules. We hope to introduce the luxI gene into Escherichia coli to produce AHL signaling molecules. (AHL is a class of signaling molecules in which the luxI gene differs from the luxR gene in different species of bacteria. The binding of signal molecules to receptor proteins is species-specific which can prevent interference from other species. However, <i>E. coli</i> is an exception that can sense all kinds of AHL signaling molecules).<br>
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The constituent produces AHL: <partinfo>BBa_K3447108</partinfo><br>
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==<b>Design</b>==
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===Design Notes===
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In order not to increase restriction sites, we modified the natural gene and added some nonsense mutations, and other than that there is no need to pay attention.<br><br>
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===Source===
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We get the sequence data on GenBank and we ordered the part DNA from a synthesis company.
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<partinfo>BBa_K3447000 parameters</partinfo>
 
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===References===

Latest revision as of 10:39, 27 October 2020

luxI, an autoinducer synthetase for AHL


LuxI is the synthetase of AHL from Vibrio fischeri.

Usage and Biology

LuxI gene is an important gene for gram-negative bacteria to conduct quorum sensing communication. It is responsible for the synthesis of AHL signaling molecules. AHL, an acyl hyperserine lactone, is the main signal molecule for gram-negative bacteria to communicate quorum sensing information. E. coli is a typical Gram-negative bacterium, but its quoral-sensing system 1 is not complete. It lacks the luxI gene, so it cannot synthesize AHL synthase and thus AHL signal molecules. We hope to introduce the luxI gene into Escherichia coli to produce AHL signaling molecules. (AHL is a class of signaling molecules in which the luxI gene differs from the luxR gene in different species of bacteria. The binding of signal molecules to receptor proteins is species-specific which can prevent interference from other species. However, E. coli is an exception that can sense all kinds of AHL signaling molecules).
The constituent produces AHL: BBa_K3447108

Design

Design Notes

In order not to increase restriction sites, we modified the natural gene and added some nonsense mutations, and other than that there is no need to pay attention.

Source

We get the sequence data on GenBank and we ordered the part DNA from a synthesis company.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References