Difference between revisions of "Part:BBa K3328017:Experience"
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===Applications of BBa_K3328017===
 
===Applications of BBa_K3328017===
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We combined the 3WJ switch and toehold switch to realize the IMPLY Boolean calculation. When no trigger expressed, this logic gate just likes a 3WJ switch. When trigger A expressed, the trigger will bind to the switch RNA. The binding allows for a branch migration process, exposing AUG and RBS for translation initiation. When trigger B expressed, the trigger will bind to 3WJ switch RNA. The resulting trigger–switch complex has a stable 3WJ structure that effectively sequesters the RBS and start codon within the loop and stem of the switch RNA, respectively, and strongly represses translation. When trigger A and B both expressed, the binding of trigger RNA to the toehold sequence allow the RNA polymerase binds to the former RBS and break open 3WJ stable hairpin.
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https://2020.igem.org/wiki/images/thumb/5/52/T--OUC-China--part_IMPLY1.jpg/800px-T--OUC-China--part_IMPLY1.jpg
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The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 10:34, 27 October 2020


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Applications of BBa_K3328017

800px-T--OUC-China--part_IMPLY1.jpg

The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).

User Reviews

UNIQd6e97e2ba313a2a7-partinfo-00000000-QINU UNIQd6e97e2ba313a2a7-partinfo-00000001-QINU