Difference between revisions of "Part:BBa K3447002"

 
 
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<partinfo>BBa_K3447002 short</partinfo>
 
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CvaC produce ColV, which is also called colicin V, a type of bacteriocin secreted by E. coli. Besides, it has a killing effect on other closely related bacteria that cannot secrete specific colicin immunity protein (synthesized by cvi gene).
 
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We will use cvaC-cvi to express ColV and immunity protein, thereby killing "enemy".
 
  
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Axh43 gene, also called xynD, is derived from <i>Bacillus subtilis</i> 168 and belongs to the GH43 hydrolase family. It can specifically hydrolyze the Arabinose residues on the O-2 xylan or o-3 xylan, and assist the hydrolysis of the xylan.
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===Usage and Biology===
 
===Usage and Biology===
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In our project, we use this part together with xynA gene  <partinfo>BBa_K1175005</Partinfo> to hydrolyze xylan and produce arabinose. For more information, please check out our part <partinfo>BBa_K3447102</Partinfo>.
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==<b>Design</b>==
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===Design Notes===
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There is no need to pay attention.<br>
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===Source===
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This gene is derived from <i>Bacillus subtilis</i> 168, we get the genomic sequence by NCBI.<br>
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We ordered the part DNA from a synthesis company.<br>
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<partinfo>BBa_K3447002 parameters</partinfo>
 
<partinfo>BBa_K3447002 parameters</partinfo>
 
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===References===

Latest revision as of 10:20, 27 October 2020


xynD, which produces xylanase


Axh43 gene, also called xynD, is derived from Bacillus subtilis 168 and belongs to the GH43 hydrolase family. It can specifically hydrolyze the Arabinose residues on the O-2 xylan or o-3 xylan, and assist the hydrolysis of the xylan.


Usage and Biology

In our project, we use this part together with xynA gene BBa_K1175005 to hydrolyze xylan and produce arabinose. For more information, please check out our part BBa_K3447102.

Design

Design Notes

There is no need to pay attention.

Source

This gene is derived from Bacillus subtilis 168, we get the genomic sequence by NCBI.
We ordered the part DNA from a synthesis company.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 712



References