Difference between revisions of "Part:BBa K3633017"
Line 3: | Line 3: | ||
<partinfo>BBa_K3633017 short</partinfo> | <partinfo>BBa_K3633017 short</partinfo> | ||
− | - | + | ==Description== |
+ | This is a constitutive promoter. iGEM20_Shanghai_SFLS_SPBS borrowed it from iGEM19_GreatBay_SZ, and it was used to express TnaA and FMO enzymes to synthesize indigo. It has worked in E. coli DH5α. | ||
+ | |||
+ | ==Experiment and Results== | ||
+ | ===Successful production of indigo in E.coli DH5α=== | ||
+ | ===Successful production in E. coli DH5α=== | ||
+ | |||
+ | We successfully used this promoter to synthesize indigo in E. coli DH5α. We added 100 mg/L L-Tryptophan in one shake flask and no substrate in another. Both shake flasks successfully produced indigo, and the one with the substrate produced more pigments than the other.<br> | ||
+ | |||
+ | [[File:T--Shanghai SFLS SPBS--indigo.png|600px|center|thumb|Figure 2. (A) Production of indigo in E. coli DH5α, with and without substrate, at 37℃ in 72 h. (B) Production of indigo from 0-60 h.]] | ||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 10:12, 27 October 2020
sp2 promoter
Description
This is a constitutive promoter. iGEM20_Shanghai_SFLS_SPBS borrowed it from iGEM19_GreatBay_SZ, and it was used to express TnaA and FMO enzymes to synthesize indigo. It has worked in E. coli DH5α.
Experiment and Results
Successful production of indigo in E.coli DH5α
Successful production in E. coli DH5α
We successfully used this promoter to synthesize indigo in E. coli DH5α. We added 100 mg/L L-Tryptophan in one shake flask and no substrate in another. Both shake flasks successfully produced indigo, and the one with the substrate produced more pigments than the other.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]