Difference between revisions of "Part:BBa K3447101"

Latest revision as of 10:05, 27 October 2020

RelE, a kind of toxin protein

RelE is from Escherichia coli str. K-12, encoding RelE toxin.

In our project, we use this part to achieve the response of blue light and inhibit the growth of bacteria.

Arabinose induced RelE expression: BBa_K3447104

AHL induced RelE expression: BBa_K3447106

We added some synonymous mutations to avoid part rules.

We found this sequence data in GenBank.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 467

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3447101 short</partinfo>
-RelE is from <i>Escherichia coli str. K-12</i>, encoding RelE toxin.
+RelE is from <i>Escherichia coli</i> str. K-12, encoding RelE toxin.
 ===Usage and Biology===
 In our project, we use this part to achieve the response of blue light and inhibit the growth of bacteria.<br>
 Arabinose induced RelE expression: <partinfo>BBa_K3447104</partinfo><br>
-AHL induced RelE expression:  <partinfo>BBa_K3447106</partinfo><br>
+AHL induced RelE expression: <partinfo>BBa_K3447106</partinfo><br>
+==<b>Design</b>==
+===Design Notes===
+We added some synonymous mutations to avoid part rules.<br>
+===Source===
+We found this sequence data in GenBank.<br>
+===References===
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>