Difference between revisions of "Part:BBa K3328014"
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| − | 1 | + | two input trigger of XOR1 logic gate to perform the truth table |
| + | ===Design=== | ||
| + | The XOR is inspired by the NIMPLY gate, consisting of a toehold switch and two triggers. The trigger’s core sequence is the same and at the triggers’ both ends there are the nucleotide-binding domains. When input one of these triggers, the switch can turn on. Moreover, when input these two triggers simultaneously, they can pair together and form a ring in the middle. As a result, the switch will still be in the OFF state. | ||
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| + | https://2020.igem.org/wiki/images/thumb/9/99/T--OUC-China--design_lunbo_xor.jpg/799px-T--OUC-China--design_lunbo_xor.jpg | ||
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| + | ===Result=== | ||
| + | https://2020.igem.org/wiki/images/thumb/7/70/T--OUC-China--part_XOR1.jpg/800px-T--OUC-China--part_XOR1.jpg | ||
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| + | The left figure shows that when INPUT A and INPUT B are both 0 or 1, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other two groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9). | ||
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Latest revision as of 09:36, 27 October 2020
triggers of XOR gate (XOR1)
two input trigger of XOR1 logic gate to perform the truth table
Design
The XOR is inspired by the NIMPLY gate, consisting of a toehold switch and two triggers. The trigger’s core sequence is the same and at the triggers’ both ends there are the nucleotide-binding domains. When input one of these triggers, the switch can turn on. Moreover, when input these two triggers simultaneously, they can pair together and form a ring in the middle. As a result, the switch will still be in the OFF state.
Result
The left figure shows that when INPUT A and INPUT B are both 0 or 1, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other two groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]