Difference between revisions of "Part:BBa K3329000"
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| − | This is a sub part in the | + | This is a sub part in the SpyTag003-SpyCatcher003 system. It should be used with SpyCatcher003. It's noteworthy that, in our Protein Crosslinking design, we intended to link a triplication of SpyTag003 through elastin-like polypeptide. Moreover, SpyTag003 was expressed in both P. Pastoris and E. coli, respectively. Therefore, codon optimization must be applied in order to avoid homologous recombination and fit the chassis organism. As a result, we synthesized 9 versions of genes coding the same SpyTag003 sequence based on the codon optimization of P. Pastoris and 3 versions for the codon optimization of E. coli. Names for those 12 versions of CDS is documented from SpyTag003 optimized V1 to SpyTag003 optimized V12, in which the SpyTag003 optimized V1-V6 are for P. Pastoris, and SpyTag003 optimized V7-V12 are for E. coli. |
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| + | We also built a model calculating the optimized ratio of SpyCatcher003 to SpyTag003 for gel formation. See more details in our Engineering Success webpage: https://2020.igem.org/Team:BNDS_China/Engineering | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 09:26, 27 October 2020
SpyTag003 optimized V1
This is a sub part in the SpyTag003-SpyCatcher003 system. It should be used with SpyCatcher003. It's noteworthy that, in our Protein Crosslinking design, we intended to link a triplication of SpyTag003 through elastin-like polypeptide. Moreover, SpyTag003 was expressed in both P. Pastoris and E. coli, respectively. Therefore, codon optimization must be applied in order to avoid homologous recombination and fit the chassis organism. As a result, we synthesized 9 versions of genes coding the same SpyTag003 sequence based on the codon optimization of P. Pastoris and 3 versions for the codon optimization of E. coli. Names for those 12 versions of CDS is documented from SpyTag003 optimized V1 to SpyTag003 optimized V12, in which the SpyTag003 optimized V1-V6 are for P. Pastoris, and SpyTag003 optimized V7-V12 are for E. coli.
We also built a model calculating the optimized ratio of SpyCatcher003 to SpyTag003 for gel formation. See more details in our Engineering Success webpage: https://2020.igem.org/Team:BNDS_China/Engineering
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Functional Parameters