Difference between revisions of "Part:BBa K3526004"
 
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__NOTOC__
 
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<partinfo>BBa_K3526004 short</partinfo>
 
<partinfo>BBa_K3526004 short</partinfo>
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<partinfo>BBa_K3526004 SequenceAndFeatures</partinfo>
  
 
A transcription factor, atzR, which can sense the presence of cyanuric acid.
 
A transcription factor, atzR, which can sense the presence of cyanuric acid.
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[[File:T--Shanghai city-BBa_K3526004 fig 1.png|500px|thumb|center|Figure 3]]
 
[[File:T--Shanghai city-BBa_K3526004 fig 1.png|500px|thumb|center|Figure 3]]
 
                                                                
 
                                                                
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The results of the experiment show that with the presence of part BBa_K3526004 and BBa_K3526005, pseudomonas putida KT2440 can detect cyanuric acid.
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The results of the experiment show that with the presence of part BBa_K3526004 and BBa_K3526005, Pseudomonas putida KT2440 can detect cyanuric acid.

Latest revision as of 08:34, 27 October 2020

tac pro-atzR-T4 ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1026
    Illegal AgeI site found at 864
  • 1000
    COMPATIBLE WITH RFC[1000]

A transcription factor, atzR, which can sense the presence of cyanuric acid.


Contribution

BBa_K3526004 is a composite part that contains a special transcription factor AtzR. This part should be used with the part BBa_K3526005. Under the presence of cyanuric acid (CYA), the AtzR can bind CYA and regulate the promoter PatzD. As a result, the GFP is expressed and emit green fluorescence.

Figure 1
Figure 2

Engineering Success

To test the function of part BBa_K3526004, we co-transform the part BBa_K3526004 and part BBa_K3526005 into Pseudomonas putida KT2440. After reading related articles, we choose 0μM, 50μM, and 100μM as the detection concentrations of CYA. After preliminary analysis, we obtained Figure 3.

Figure 3

The results of the experiment show that with the presence of part BBa_K3526004 and BBa_K3526005, Pseudomonas putida KT2440 can detect cyanuric acid.