Difference between revisions of "Part:BBa K3506099"

 
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<partinfo>BBa_K3506099 short</partinfo>
 
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It is generally accepted that GAPDH promoter (pGAP) is considered to be a strong constitutive promoter which is used by RNA polymerase II.
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Generally, <i>GAPDH</i> promoter can drive the expression of glyceraldehyde-3-phosphate dehydrogenase gene in many species.
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<!-- Add more about the biology of this part here
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3506099 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3506099 SequenceAndFeatures</partinfo>
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<b><font size"3">Biology and Usage</font></b>
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<i>GAPDH</i> mRNA accounts for 2–5% of the poly(A) RNA in yeasts, suggesting that GAPDH promoter is a highly active promoter. In our project, <i>GAPDH</i> promoter is used to effectively transcribe the genes that contains <i>U6</i> promoter and gRNA. General vectors have been designed that rely on homologous <i>GAPDH</i> promoter regions to drive the expressions of heterologous genes in yeasts and other filamentous fungi.
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<b><font size"3">Reference</font></b>
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[1]Jia Y, Li S, Allen G, Feng S, Xue L. A novel glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter for expressing transgenes in the halotolerant alga Dunaliella salina. Curr Microbiol. 2012 May;64(5):506-13. doi: 10.1007/s00284-012-0102-y. Epub 2012 Feb 28. PMID: 22371187.
  
  

Revision as of 08:32, 27 October 2020


GAPDH promoter

Generally, GAPDH promoter can drive the expression of glyceraldehyde-3-phosphate dehydrogenase gene in many species.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 436


Biology and Usage

GAPDH mRNA accounts for 2–5% of the poly(A) RNA in yeasts, suggesting that GAPDH promoter is a highly active promoter. In our project, GAPDH promoter is used to effectively transcribe the genes that contains U6 promoter and gRNA. General vectors have been designed that rely on homologous GAPDH promoter regions to drive the expressions of heterologous genes in yeasts and other filamentous fungi.

Reference

[1]Jia Y, Li S, Allen G, Feng S, Xue L. A novel glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter for expressing transgenes in the halotolerant alga Dunaliella salina. Curr Microbiol. 2012 May;64(5):506-13. doi: 10.1007/s00284-012-0102-y. Epub 2012 Feb 28. PMID: 22371187.