Difference between revisions of "Part:BBa K3378000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The sequence of this part has been optimized based on E. coli codon bias. | + | The sequence of this part has been optimized based on <i>E. coli</i> codon bias. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | The catalytic domain come from Streptococcus virus | + | The catalytic domain come from <i>Streptococcus virus</i> CI and the cell-wall binding domain come from <i>Streptococcus suis</i>. |
===References=== | ===References=== | ||
[1] Yang, Hang, et al. "A chimeolysin with extended-spectrum streptococcal host range found by an induced lysis-based rapid screening method." Scientific reports 5 (2015): 17257. | [1] Yang, Hang, et al. "A chimeolysin with extended-spectrum streptococcal host range found by an induced lysis-based rapid screening method." Scientific reports 5 (2015): 17257. | ||
| − | [2] Yang, Hang, et al. "Antibiofilm activities of a novel chimeolysin against Streptococcus mutans under physiological and cariogenic conditions." Antimicrobial agents and chemotherapy 60.12 (2016): 7436-7443. | + | [2] Yang, Hang, et al. "Antibiofilm activities of a novel chimeolysin against <i>Streptococcus mutans</i> under physiological and cariogenic conditions." Antimicrobial agents and chemotherapy 60.12 (2016): 7436-7443. |
[3] Xu, Jingjing, et al. "Activity of the chimeric lysin ClyR against common Gram-positive oral microbes and its anticaries efficacy in rat models." Viruses 10.7 (2018): 380. | [3] Xu, Jingjing, et al. "Activity of the chimeric lysin ClyR against common Gram-positive oral microbes and its anticaries efficacy in rat models." Viruses 10.7 (2018): 380. | ||
Latest revision as of 08:32, 27 October 2020
Chimeric lysin ClyR with activity against Streptococcus mutans.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 757
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 121
Illegal AgeI site found at 205
Illegal AgeI site found at 520
Illegal AgeI site found at 703 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence of this part has been optimized based on E. coli codon bias.
Source
The catalytic domain come from Streptococcus virus CI and the cell-wall binding domain come from Streptococcus suis.
References
[1] Yang, Hang, et al. "A chimeolysin with extended-spectrum streptococcal host range found by an induced lysis-based rapid screening method." Scientific reports 5 (2015): 17257.
[2] Yang, Hang, et al. "Antibiofilm activities of a novel chimeolysin against Streptococcus mutans under physiological and cariogenic conditions." Antimicrobial agents and chemotherapy 60.12 (2016): 7436-7443.
[3] Xu, Jingjing, et al. "Activity of the chimeric lysin ClyR against common Gram-positive oral microbes and its anticaries efficacy in rat models." Viruses 10.7 (2018): 380.