Difference between revisions of "Part:BBa K3523008"

 
 
(One intermediate revision by one other user not shown)
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3523008 short</partinfo>
 
<partinfo>BBa_K3523008 short</partinfo>
 +
 +
<partinfo>BBa_K3523008 SequenceAndFeatures</partinfo>
  
 
BBa_K3523008 is used to express a fusion protein, helping to display it on the cell membrane surface. The fusion protein contains superoxide dismutases (SOD) and catalase,. The SOD is a group of enzymes that catalyze the dismutation of superoxide radicals (O2&#8722;) to molecular oxygen (O2) and hydrogen peroxide (H2O2), providing cellular defense against reactive oxygen species. The function of catalase is decomposing hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide. The promoter J23116 is used to initiate transcription and translation of the fusion protein.
 
BBa_K3523008 is used to express a fusion protein, helping to display it on the cell membrane surface. The fusion protein contains superoxide dismutases (SOD) and catalase,. The SOD is a group of enzymes that catalyze the dismutation of superoxide radicals (O2&#8722;) to molecular oxygen (O2) and hydrogen peroxide (H2O2), providing cellular defense against reactive oxygen species. The function of catalase is decomposing hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide. The promoter J23116 is used to initiate transcription and translation of the fusion protein.
  
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
−
 
−
<!-- -->
 
−
<span class='h3bb'>Sequence and Features</span>
 
−
<partinfo>BBa_K3523008 SequenceAndFeatures</partinfo>
 
  
 +
===Contribution===
  
−
<!-- Uncomment this to enable Functional Parameter display
+
[[File:T--Xiamen_city-BBa_K3523008_figure 0.jpg|500px|thumb|center|figure 1]]
−
===Functional Parameters===
+
In order to eliminate ROS of the intestinal tract more efficiently and rapidly, we further designed the fusion expression of superoxide dismutase (SOD) and catalase(CAT) with membrane proteins (Ag43)^9 of E. coli, so that the enzymes responsible for superoxide degradation were displayed on the surface of the cell membrane. This strategy can promote the contact of enzymes and substrates and accelerate the process of ROS degradation by engineered bacteria theoretically. We have constructed plasmids for this purpose and will further test this hypothesis.
−
<partinfo>BBa_K3523008 parameters</partinfo>
+
[[File:T--Xiamen_city-BBa_K3523008_fig_00.png|500px|thumb|center|figure 2]]
−
<!-- -->
+

Latest revision as of 08:30, 27 October 2020


J23116-rbs-Leader sequence-SOD-rbs-katA-Membrane Protein Ag43_700


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2166
    Illegal NgoMIV site found at 2710
    Illegal NgoMIV site found at 2731
    Illegal AgeI site found at 2470
    Illegal AgeI site found at 2884
    Illegal AgeI site found at 3126
  • 1000
    COMPATIBLE WITH RFC[1000]

BBa_K3523008 is used to express a fusion protein, helping to display it on the cell membrane surface. The fusion protein contains superoxide dismutases (SOD) and catalase,. The SOD is a group of enzymes that catalyze the dismutation of superoxide radicals (O2−) to molecular oxygen (O2) and hydrogen peroxide (H2O2), providing cellular defense against reactive oxygen species. The function of catalase is decomposing hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide. The promoter J23116 is used to initiate transcription and translation of the fusion protein.


Contribution

figure 1

In order to eliminate ROS of the intestinal tract more efficiently and rapidly, we further designed the fusion expression of superoxide dismutase (SOD) and catalase(CAT) with membrane proteins (Ag43)^9 of E. coli, so that the enzymes responsible for superoxide degradation were displayed on the surface of the cell membrane. This strategy can promote the contact of enzymes and substrates and accelerate the process of ROS degradation by engineered bacteria theoretically. We have constructed plasmids for this purpose and will further test this hypothesis.

figure 2