Difference between revisions of "Part:BBa K3523007"

 
 
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<partinfo>BBa_K3523007 short</partinfo>
 
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<partinfo>BBa_K3523007 SequenceAndFeatures</partinfo>
  
 
BBa_K3523007 is used to express a fusion protein, contains superoxide dismutases (SOD) and catalase.
 
BBa_K3523007 is used to express a fusion protein, contains superoxide dismutases (SOD) and catalase.
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The promoter J23116 is used to initiate transcription and translation of the fusion protein.
 
The promoter J23116 is used to initiate transcription and translation of the fusion protein.
  
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===Contribution===
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===Usage and Biology===
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We use an existing Part: BBa_J23116 to start classical enzymes -- superoxide dismutase (SOD) and catalase(CAT) protein transcription without a terminator. The new composite Part: BBa_K3523007 can be used for topics related to the degradation of ROS in the future.
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[[File:T--Xiamen_city-BBa_K3523007_figure 0.jpg|500px|thumb|center|figure 1]]
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We transformative the new composite part into Escherichia coli Nissle 1917 to construct an engineered bacteria which will scavenge superoxide compounds (ROS) in the gut quickly and efficiently.
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<span class='h3bb'>Sequence and Features</span>
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[[File:T--Xiamen_city-BBa_K3523007_figure 00.jpg|500px|thumb|center|figure 2]]
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<partinfo>BBa_K3523007 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3523007 parameters</partinfo>
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Latest revision as of 08:29, 27 October 2020


J23116-rbs-SOD-rbs-katA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2121
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1993
  • 1000
    COMPATIBLE WITH RFC[1000]

BBa_K3523007 is used to express a fusion protein, contains superoxide dismutases (SOD) and catalase. The SOD is a group of enzymes that catalyze the dismutation of superoxide radicals (O2−) to molecular oxygen (O2) and hydrogen peroxide (H2O2), providing cellular defense against reactive oxygen species. The function of catalase is decomposing hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide. The promoter J23116 is used to initiate transcription and translation of the fusion protein.

Contribution

We use an existing Part: BBa_J23116 to start classical enzymes -- superoxide dismutase (SOD) and catalase(CAT) protein transcription without a terminator. The new composite Part: BBa_K3523007 can be used for topics related to the degradation of ROS in the future.

figure 1

We transformative the new composite part into Escherichia coli Nissle 1917 to construct an engineered bacteria which will scavenge superoxide compounds (ROS) in the gut quickly and efficiently.

figure 2