Difference between revisions of "Part:BBa K3629020:Design"

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===Design Notes===
 
===Design Notes===
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Codon optimized for expression and function in <i>Yarrowia lipolytica.</i>
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Below is the codon-optimized sequence of <i>Yarrowia lipolytica</i> TRP2 for use in any expression construct. However, we have also made our own expression construct [https://parts.igem.org/Part:BBa_K3629024 BBa_K3629024], designed to allow for optimal expression of TRP2 in <i>Yarrowia lipolytica</i> using the TEfin promoter [https://parts.igem.org/Part:BBa_K3629001 BBa_K3629001] and the XPR2 terminator [https://parts.igem.org/Part:BBa_K3629004 BBa_K3629004].
  
 
===Source===
 
===Source===
  
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From genome sequence of <i>Yarrowia lipolytica.</i>
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From genome sequence of <i>Yarrowia lipolytica</i> CDS: T01033.
  
 
===References===
 
===References===
 
1. [online] https://www.genome.jp/kegg-bin/show_pathway?yli00400 (Accessed October 26, 2020)
 
1. [online] https://www.genome.jp/kegg-bin/show_pathway?yli00400 (Accessed October 26, 2020)

Latest revision as of 07:43, 27 October 2020


Yarrowia lipolytica TRP2 Gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 943
    Illegal NgoMIV site found at 1213
    Illegal NgoMIV site found at 1294
    Illegal NgoMIV site found at 1393
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 283
    Illegal BsaI.rc site found at 544
    Illegal BsaI.rc site found at 1441


Design Notes

Below is the codon-optimized sequence of Yarrowia lipolytica TRP2 for use in any expression construct. However, we have also made our own expression construct BBa_K3629024, designed to allow for optimal expression of TRP2 in Yarrowia lipolytica using the TEfin promoter BBa_K3629001 and the XPR2 terminator BBa_K3629004.

Source

From genome sequence of Yarrowia lipolytica CDS: T01033.

References

1. [online] https://www.genome.jp/kegg-bin/show_pathway?yli00400 (Accessed October 26, 2020)