Difference between revisions of "Part:BBa K3406008:Design"

 
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<partinfo>BBa_K2323008 short</partinfo>
  
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<partinfo>BBa_K2323008 SequenceAndFeatures</partinfo>
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===Design Notes===
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We use Recombinase Polymerase Amplification to amplify a short sequence of H1N1 genome. To maximize amplification success, the primer lengths should be ~25–35 nt, and the total amplicon size should be 80–140 bp. Primers are typically designed with melting temperatures between 54 and 67 °C. In addition, because we need to do transcription experiments downstream, a T7RNA polymerase promoter was appended to the 5ʹend of the forward primers to allow T7 transcription.

Revision as of 07:12, 27 October 2020

GFP-pdt2B


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


Design Notes

We use Recombinase Polymerase Amplification to amplify a short sequence of H1N1 genome. To maximize amplification success, the primer lengths should be ~25–35 nt, and the total amplicon size should be 80–140 bp. Primers are typically designed with melting temperatures between 54 and 67 °C. In addition, because we need to do transcription experiments downstream, a T7RNA polymerase promoter was appended to the 5ʹend of the forward primers to allow T7 transcription.