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===Design Notes===
===Design Notes===
−
There is a repetitive sequence between <i>cvaA</i> and <i>cvaB</i> gene. In order not to increase restriction sites, we modified the natural gene and added some nonsense mutations, and other than that there is no need to pay attention.
+
We modified the natural gene and added some nonsense mutations, and other than that there is no need to pay attention.
===Source===
===Source===
−
We will use cvaAB to express channel proteins for the transport of ColV out of the cell membrane.<br>
+
We get the genomic sequence by NCBI and ordered the part DNA from a synthesis company.
−
This gene is derived from <i>Escherichia coli</i> (strain: A2363), we get the genomic sequence by NCBI.<br>
1. Coproduction of colicin V and lactic acid bacteria bacteriocins in lactococci and enterococci strains of biotechnological interest., <i>Journal of Applied Microbiology</i>, <b>122</b>, 1159-1167.<br>
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2. Colicin V can be produced by lactic acid bacteria.(1999), <i>Letters in Applied Microbiology</i>, <b>29</b>, 37-41.<br>
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3. Mutational Analysis of Cvab, an ABC Transporter Involved in the Secretion of Active Colicin V., Kai-Hui Wu., Ying-Hsin Hsieh., Phang C. Tai, <i>Plos One</i>, <b>7(4)</b>, e35382.<br>
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4. Purification and Characterization of Colicin V from Escherichia coli Culture Supernatants.(1994), <i>Biochemistry</i>, <b>33</b>, 6911-6917.<br>
Latest revision as of 06:57, 27 October 2020
luxI, an autoinducer synthetase for AHL
Assembly Compatibility:
10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]
Design Notes
We modified the natural gene and added some nonsense mutations, and other than that there is no need to pay attention.
Source
We get the genomic sequence by NCBI and ordered the part DNA from a synthesis company.
