Difference between revisions of "Part:BBa K3599017"

 
 
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<partinfo>BBa_K3599017 short</partinfo>
 
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Green fluorescent protein, we used its sequence as a reporting gene to measure the intensity of the expression.
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GFP variant that folds robustly even when fused to poorly folded proteins. We used its sequence as a reporting gene to measure the intensity of the expression.
  
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===Usage and Biology===
 
  
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===Sequence and Features===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3599017 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3599017 SequenceAndFeatures</partinfo>
  
  
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===Reference===
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===Functional Parameters===
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Pédelacq, J., Cabantous, S., Tran, T. et al. Engineering and characterization of a superfolder green fluorescent protein. <i>Nat Biotechnol</i> 24, 79–88 (2006). [https://doi.org/10.1038/nbt1172 https://doi.org/10.1038/nbt1172]
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<partinfo>BBa_K3599017 parameters</partinfo>
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Latest revision as of 06:55, 27 October 2020


superfolder GFP

GFP variant that folds robustly even when fused to poorly folded proteins. We used its sequence as a reporting gene to measure the intensity of the expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


Reference

Pédelacq, J., Cabantous, S., Tran, T. et al. Engineering and characterization of a superfolder green fluorescent protein. Nat Biotechnol 24, 79–88 (2006). https://doi.org/10.1038/nbt1172