Difference between revisions of "Part:BBa K3457058"
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<partinfo>BBa_K3457058 short</partinfo> | <partinfo>BBa_K3457058 short</partinfo> | ||
| + | <p> This biological part is designed to sense uric acid and express CAHS 106094 at the same time. CAHS 106094 is used to protect the bacteria during storage.</p> | ||
| − | a | + | ===Contribution=== |
| + | <h3><b>Group: QHFZ-China iGEM 2020</b></h3> | ||
| + | <h3><b>Author: Yixian Yang</b></h3> | ||
| + | <p>The part is a combination of [https://parts.igem.org/Part:BBa_K3007029 BBa_K3007029] PhucR-dsRed-Pcp6-HucR & YgfU (uric acid sensor) and [https://parts.igem.org/Part:BBa_K3457046 BBa_K3457046] J23100-Olac-RBS-CAHS 106094. The design and reslt are as below. However, there is something wrong. The constitutive expression of the reporter, dsRed, was over high. The possible reason is: (1) the construction influence the CP6 promoter, so that the expression of the repressor, HucR, was insufficient; (2) CAHS 106094 affected the uric acid sensor. Further study should be taken.</p> | ||
| + | [[File:T--QHFZ-China--ua.png|600px|thumb|left|Figure 1. Design and result.]] | ||
| + | |||
| + | <p style="clear:left;"></p> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 03:18, 27 October 2020
UA sensor-dsRed-J23100-CAHS 106094
This biological part is designed to sense uric acid and express CAHS 106094 at the same time. CAHS 106094 is used to protect the bacteria during storage.
Contribution
Group: QHFZ-China iGEM 2020
Author: Yixian Yang
The part is a combination of BBa_K3007029 PhucR-dsRed-Pcp6-HucR & YgfU (uric acid sensor) and BBa_K3457046 J23100-Olac-RBS-CAHS 106094. The design and reslt are as below. However, there is something wrong. The constitutive expression of the reporter, dsRed, was over high. The possible reason is: (1) the construction influence the CP6 promoter, so that the expression of the repressor, HucR, was insufficient; (2) CAHS 106094 affected the uric acid sensor. Further study should be taken.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 3263
Illegal EcoRI site found at 1399
Illegal EcoRI site found at 3216
Illegal XbaI site found at 3113
Illegal SpeI site found at 917
Illegal PstI site found at 140
Illegal PstI site found at 510
Illegal PstI site found at 3210 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1399
Illegal EcoRI site found at 3216
Illegal EcoRI site found at 3263
Illegal NheI site found at 1085
Illegal NheI site found at 3291
Illegal NheI site found at 3314
Illegal NheI site found at 4225
Illegal SpeI site found at 917
Illegal PstI site found at 140
Illegal PstI site found at 510
Illegal PstI site found at 3210
Illegal NotI site found at 3269 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1399
Illegal EcoRI site found at 3216
Illegal EcoRI site found at 3263
Illegal BglII site found at 852
Illegal BglII site found at 3256
Illegal BamHI site found at 158
Illegal BamHI site found at 2266
Illegal BamHI site found at 3130
Illegal BamHI site found at 3205 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 3263
Illegal EcoRI site found at 1399
Illegal EcoRI site found at 3216
Illegal XbaI site found at 3113
Illegal SpeI site found at 917
Illegal PstI site found at 140
Illegal PstI site found at 510
Illegal PstI site found at 3210 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 3263
Illegal EcoRI site found at 1399
Illegal EcoRI site found at 3216
Illegal XbaI site found at 3113
Illegal XbaI site found at 3278
Illegal SpeI site found at 917
Illegal PstI site found at 140
Illegal PstI site found at 510
Illegal PstI site found at 3210
Illegal AgeI site found at 2084 - 1000COMPATIBLE WITH RFC[1000]