Difference between revisions of "Part:BBa K3595006"

Revision as of 02:36, 27 October 2020

Mutant-cysE-5

cysE-5 is a mutant of cysE gene, which is obtained by substituting GTC (Val), GAT (Asp) at the 95th and 96th positions for AGA (Arg), CCC (Pro).In the pathway of converting hydrogen sulfide to L-cysteine, L-serine and Acetyl-CoA form O-acetylserine catalyzed by L-serine O-acetyltransferase (SAT). SAT is encoded by gene cysE, and its feedback is inhibited by the final product L-cysteine . In order to induce overproduction of L-cysteine, we need to develop a mutant gene of cysE which will code for feedback inhibition-insensitive SAT.We tried to construct different mutants through site mutation, and cysE-5 is one of the different mutants.

Sequence and Features