Difference between revisions of "Part:BBa K3629005"
(→Design) |
(→Usage and Biology) |
||
| Line 7: | Line 7: | ||
__TOC__ | __TOC__ | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | |||
| + | <i>Yarrowia lipolytica</i> is an emerging chassis in the molecular biology community. Its unique metabolic properties and efficient protein production and secretion mechanisms make it a desirable chassis for heterologous protein expression/secretion. In fact, it has been shown to have better secretory mechanisms than <i>Saccharomyces cerevisiae</i> (1). Therefore, using these chassis to secrete cellulase enzymes- which are enzymes that require high levels of secretion, is well suited. | ||
===Design=== | ===Design=== | ||
Revision as of 23:20, 26 October 2020
Modified Penicillium funiculosum CBHI with 6X His tag
Temperature and pH optimized cellobiohydrolase I coding sequence from Penicillium funiculosum with 6x HIS tag.
Usage and Biology
Yarrowia lipolytica is an emerging chassis in the molecular biology community. Its unique metabolic properties and efficient protein production and secretion mechanisms make it a desirable chassis for heterologous protein expression/secretion. In fact, it has been shown to have better secretory mechanisms than Saccharomyces cerevisiae (1). Therefore, using these chassis to secrete cellulase enzymes- which are enzymes that require high levels of secretion, is well suited.
Design
The native signal peptide from P. funiculosum was removed so it would not interfere with fused secretion tags native to Y. lipolytica.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 564
Illegal BamHI site found at 1048 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 46
- 1000COMPATIBLE WITH RFC[1000]