Difference between revisions of "Part:BBa K3629002"
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EXP promoter is a native <i>Yarrowia lipolytica</i> promoter for the EXP gene. Is one of the strongest constitutive promoters found in the organism. EXP promoter is often used for the expression of heterologous proteins in <i>Y. lipolytica</i> due to its high transcriptional level (1).
 
EXP promoter is a native <i>Yarrowia lipolytica</i> promoter for the EXP gene. Is one of the strongest constitutive promoters found in the organism. EXP promoter is often used for the expression of heterologous proteins in <i>Y. lipolytica</i> due to its high transcriptional level (1).
  
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This promoter was used in the TrEGII expression construct [https://parts.igem.org/Part:BBa_K3629017 (BBa_K3629017)] as this promoter, in combination with the XPR2 signal peptide [https://parts.igem.org/Part:BBa_K36290000(BBa_K3629000)], has  been shown to secrete high levels of TrEGII in <i>Y. lipolytica</i> (up to 132mg/L) (2). This promoter is thought not to be as strong as TEFin [https://parts.igem.org/Part:BBa_K3629001(BBa_K3619001)] promoter (2) but has approximately 1.2x greater activity than the wild-type TEF1 [https://parts.igem.org/Part:BBa_K2117000 (BBa_K211700)]  promoter (2).
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This promoter was used in the TrEGII expression construct [https://parts.igem.org/Part:BBa_K3629017 (BBa_K3629017)] as this promoter, in combination with the XPR2 signal peptide [https://parts.igem.org/Part:BBa_K36290000 (BBa_K3629000)], has  been shown to secrete high levels of TrEGII in <i>Y. lipolytica</i> (up to 132mg/L) (2). This promoter is thought not to be as strong as TEFin [https://parts.igem.org/Part:BBa_K3629001 (BBa_K3619001)] promoter (2) but has approximately 1.2x greater activity than the wild-type TEF1 [https://parts.igem.org/Part:BBa_K2117000 (BBa_K211700)]  promoter (2).
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===Sequence and Features===
 
===Sequence and Features===

Revision as of 23:14, 26 October 2020


Yarrowia lipolytica EXP Promoter

Promoter from the Yarrowia lipolytica EXP gene (an extracellular protease).

Usage and Biology

EXP promoter is a native Yarrowia lipolytica promoter for the EXP gene. Is one of the strongest constitutive promoters found in the organism. EXP promoter is often used for the expression of heterologous proteins in Y. lipolytica due to its high transcriptional level (1).

This promoter was used in the TrEGII expression construct (BBa_K3629017) as this promoter, in combination with the XPR2 signal peptide (BBa_K3629000), has been shown to secrete high levels of TrEGII in Y. lipolytica (up to 132mg/L) (2). This promoter is thought not to be as strong as TEFin (BBa_K3619001) promoter (2) but has approximately 1.2x greater activity than the wild-type TEF1 (BBa_K211700) promoter (2).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1025
    Illegal SpeI site found at 602
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 663
    Illegal SpeI site found at 602
    Illegal SpeI site found at 1026
    Illegal PstI site found at 1040
    Illegal NotI site found at 7
    Illegal NotI site found at 1033
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1026
    Illegal SpeI site found at 602
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 602
    Illegal SpeI site found at 1026
    Illegal PstI site found at 1040
  • 1000
    COMPATIBLE WITH RFC[1000]

References

1. Blazeck, J., Liu, L., Redden, H., & Alper, H. (2011). Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Applied and environmental microbiology, 77(22), 7905–7914. https://doi.org/10.1128/AEM.05763-11

2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6333634/