Difference between revisions of "Part:BBa K3453164"

Latest revision as of 22:29, 26 October 2020

Rosewood DmTrnL-UAA Trigger 2.1 transcription unit

This part is a transcription unit of the Rosewood DmTrnL-UAA Trigger 2.1 (BBa_K3453064) for sequence-based detection of rosewood. The transcription is controlled by the T7 promoter (BBa_K2150031) and the strong SBa_000587 synthetic terminator (BBa_K3453000).

Usage and Biology

A toehold switch is an RNA–based device containing a ribosome binding site (RBS) and an ATG start codon embedded in a hairpin structure that blocks translation initiation [1]. The hairpin can be unfolded upon binding of a trigger RNA therefore exposing the RBS and the ATG start codon permitting translation of the reporter protein (Figure 1).

Figure 1. Toehold switches principle.

This part is a transcription unit of BBa_K3453064 which is a trigger for the Rosewood DmTrnL-UAA Toehold Switch 2.1 (BBa_K3453054).

The functionality of this part could not be assessed because the corresponding toehold switch proved to be not functional: a readable output was generated even in the absence of rosewood RNA.

Full results are available on the BBa_K3453154 page in the registry.

References

[1] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.

Sequence and Features