Difference between revisions of "Part:BBa K3425070"

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<partinfo>BBa_K3425070 short</partinfo>
 
<partinfo>BBa_K3425070 short</partinfo>
  
This composite part serves for display of nanobodies on the outer surface of <i>E. coli</i>. It is designed as a modular system in which nanobody module (in this case [[Part:BBa_K3425043|<partinfo>BBa_K3425043</partinfo>]]) can be easily exchanged for nanobody of different specificity. All components are tailored for <html><a href="https://parts.igem.org/Help:Standards/Assembly/Type_IIS">iGEM Type IIS assembly standard</a></html>.
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This composite part serves for display of nanobodies on the outer surface of <i>E. coli</i>. It is designed as a modular system of, in which the nanobody module (in this case [[Part:BBa_K3425043|<partinfo>BBa_K3425043</partinfo>]]) can be easily exchanged for nanobody of different specificity. All components are tailored for <html><a href="https://parts.igem.org/Help:Standards/Assembly/Type_IIS">iGEM Type IIS assembly standard</a></html>.
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<br>[[Part:BBa_K3425042|<partinfo>BBa_K3425042</partinfo>]] is a signal peptide directing the fusion protein to the periplasm.
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<br>[[Part:BBa_K3425043|<partinfo>BBa_K3425043</partinfo>]] is the nanobody specific for <i>Mycobacterium tuberculosis</i> biomarker HSP 16.3 ([[Part:BBa_K3425041|<partinfo>BBa_K3425041</partinfo>]])
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<br>[[Part:BBa_K3425044|<partinfo>BBa_K3425044</partinfo>]] can be used as an outer membrane display system for <i>E. coli</i>
  
  

Revision as of 22:26, 26 October 2020


Modular outer membrane display construct for nanobody specific to HSP biomarker

This composite part serves for display of nanobodies on the outer surface of E. coli. It is designed as a modular system of, in which the nanobody module (in this case BBa_K3425043) can be easily exchanged for nanobody of different specificity. All components are tailored for iGEM Type IIS assembly standard.


BBa_K3425042 is a signal peptide directing the fusion protein to the periplasm.
BBa_K3425043 is the nanobody specific for Mycobacterium tuberculosis biomarker HSP 16.3 (BBa_K3425041)
BBa_K3425044 can be used as an outer membrane display system for E. coli


Part number Name Description
BBa_K3425042 pelB signal peptide
BBa_K3425043 B-F10 HSP Nanobody
BBa_K3425044 ehaA Transmembrane domain


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1036
    Illegal AgeI site found at 625
  • 1000
    COMPATIBLE WITH RFC[1000]