Difference between revisions of "Part:BBa K133019"

Revision as of 22:06, 29 October 2008

CMV-CF213-multiHP-CF215-RGD-Histop

We have used CMV promotor, because it is promotor for constitutive expression in mammalian cells.

The rest of the part consists of:

CF213, which is the N-terminal portion of chimeric fusion protein; it contains 176 AA of the N-terminal portion of E. coli flagellin + N-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 213 AA; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)

CF215, which is the C-terminal portion of chimeric fusion protein; it contains the C-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 215 AA + 99 AA of C-terminal portion from E. coli flagellin; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)

We also designed synthetic multiepitope, comprising the H. pylori urease B epitope, a vacuolating cytotoxin A (Vac) epitope and an adhesin A (HpaA) epitope. B-cell epitopes were designed using available 3D models or structures, which enabled more accurate prediction. Multiepitope is a synthetic gene orderd from GeneArt.

Since the protein was designed to be produced, we additionally added RGD-Hisstop tag (for the part description and implication follow: [1]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2406
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1963
Illegal BamHI site found at 2474
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 961
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2757

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K133019 short</partinfo>
-CMV-CF213-MULTI-CF215-RGD-HIS-STOP
+We have used CMV promotor, because it is promotor for constitutive expression in mammalian cells.
+The rest of the part consists of:
+CF213, which is the N-terminal portion of chimeric fusion protein; it contains 176 AA of the N-terminal portion of E. coli flagellin + N-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 213 AA; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)
+CF215, which is the C-terminal portion of chimeric fusion protein; it contains the C-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 215 AA + 99 AA of C-terminal portion from E. coli flagellin; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)
+We also designed synthetic multiepitope, comprising the H. pylori urease B epitope, a vacuolating cytotoxin A (Vac) epitope and an adhesin A (HpaA) epitope. B-cell epitopes were designed using available 3D models or structures, which enabled more accurate prediction. Multiepitope is a synthetic gene orderd from GeneArt.
+Since the protein was designed to be produced, we additionally added RGD-Hisstop tag (for the part description and implication follow: [1]