Difference between revisions of "Part:BBa K3425070"
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<partinfo>BBa_K3425070 short</partinfo>
 
<partinfo>BBa_K3425070 short</partinfo>
  
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Outer membrane construct with anti-HSP nanobody
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This composite part serves for display of nanobodies on the outer surface of <i>E. coli</i>. It is designed as a modular system in which nanobody module (in this case ) can be easily exchanged for nanobody tailored to the <a href="https://parts.igem.org/Help:Standards/Assembly/Type_IIS">iGEM Type IIS assembly</a>
  
  

Revision as of 22:16, 26 October 2020


Modular outer membrane display construct for nanobody specific to HSP biomarker

This composite part serves for display of nanobodies on the outer surface of E. coli. It is designed as a modular system in which nanobody module (in this case ) can be easily exchanged for nanobody tailored to the <a href="https://parts.igem.org/Help:Standards/Assembly/Type_IIS">iGEM Type IIS assembly</a>


Part number Name Description
BBa_K3425042 pelB signal peptide
BBa_K3425043 B-F10 HSP Nanobody
BBa_K3425044 ehaA Transmembrane domain


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1036
    Illegal AgeI site found at 625
  • 1000
    COMPATIBLE WITH RFC[1000]