Difference between revisions of "Part:BBa K3411020"
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Lambert iGEM’s novel NarP system BBa_K3411020 is also modeled after <i>E.coli’s</i> natural nitrite sensor in the NAR Operon. Similarly to NarL, the promoter BBa_J23106, a constitutive Anderson Promoter, produces TetR, which represses the PtetO promoter, producing NarP. By regulating the amount of aTc, the molecule that inhibits TetR, our lab can control NarP’s expression, the product of the PtetO promoter. NarQ, the native membrane-bound protein, senses the amount of nitrate and/or nitrite in the cell: In the presence of nitrite and/or nitrate, NarQ will phosphorylate NarP, activating it, but if there is no nitrite and/or nitrate present, NarQ will not phosphorylate NarP. If NarP is phosphorylated, it will activate the nirB promoter, producing GFP. | Lambert iGEM’s novel NarP system BBa_K3411020 is also modeled after <i>E.coli’s</i> natural nitrite sensor in the NAR Operon. Similarly to NarL, the promoter BBa_J23106, a constitutive Anderson Promoter, produces TetR, which represses the PtetO promoter, producing NarP. By regulating the amount of aTc, the molecule that inhibits TetR, our lab can control NarP’s expression, the product of the PtetO promoter. NarQ, the native membrane-bound protein, senses the amount of nitrate and/or nitrite in the cell: In the presence of nitrite and/or nitrate, NarQ will phosphorylate NarP, activating it, but if there is no nitrite and/or nitrate present, NarQ will not phosphorylate NarP. If NarP is phosphorylated, it will activate the nirB promoter, producing GFP. | ||
Revision as of 21:57, 26 October 2020
NarP Nitrate/Nitrite Sensor
Lambert iGEM’s novel NarP system BBa_K3411020 is also modeled after E.coli’s natural nitrite sensor in the NAR Operon. Similarly to NarL, the promoter BBa_J23106, a constitutive Anderson Promoter, produces TetR, which represses the PtetO promoter, producing NarP. By regulating the amount of aTc, the molecule that inhibits TetR, our lab can control NarP’s expression, the product of the PtetO promoter. NarQ, the native membrane-bound protein, senses the amount of nitrate and/or nitrite in the cell: In the presence of nitrite and/or nitrate, NarQ will phosphorylate NarP, activating it, but if there is no nitrite and/or nitrate present, NarQ will not phosphorylate NarP. If NarP is phosphorylated, it will activate the nirB promoter, producing GFP.
Lambert iGEM plans on cloning and characterizing our NarP composite part in the coming year as a part of our two-year project, AgroSense.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2000
Illegal NheI site found at 2023 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2727
Illegal AgeI site found at 1371 - 1000COMPATIBLE WITH RFC[1000]